WebVazyme LAmp DNA Polymerase is a mixed enzyme of Taq DNA Polymerase and a protein containing 3'→5' exonuclease activity (proofreading activity). The fidelity is 6-fold higher than Taq DNA Polymerase. With a specially optimized buffer system, Vazyme LAmp DNA Polymerase is suitable for Long-range PCR (up to 21 kb when using the genome as a ... WebMar 9, 2015 · 1.4 Prepare 40 mL of 1X Click-iT® HPG reaction buffer by transferring all of the solution (4 mL) in the Component C bottle to 36 mL of deionized water. Rinse the Component C bottle with some of the diluted Click-iT® HPG Reaction buffer to ensure the transfer of all of the 10X concentrate.
Click Chemistry: new protocol for the labeling and …
WebAfter use, store any remaining 1X Click-iT® cell reaction buffer at 2–6˚C. When stored as directed, the 1X Click-iT® cell reaction buffer is stable for 6 months. Preparing Click … WebClick-iT™ EdU reaction additive (Component F) into deionized water. 4. Click-iT™ wash buffer - For each 96-well plate being tested, prepare 1X Click-iT™ EdU wash buffer by adding 0.5 mL of the 200X Click-iT™ wash buffer additive (Component G) into 99.5 mL of PBS. The resulting of amount of 1X Click-iT™ EdU wash buffer bubbles car wash selby
Invitrogen™ Click-iT™ Protein Reaction Buffer Kit
WebJun 5, 2024 · Component F: Click-iT ® Reaction Rise Buffer, containing 2 mM sodium azide. Component G: NuclearMask TM Blue Stain, 2,000× concentrate in water Chemicals and reagents Dulbecco′s Phosphate Buffered Saline (PBS) (Sigma, catalog number: D5773) Triton X-100 (Sigma, catalog number: T8787) Bovine serum albumin (BSA) (Sigma, … WebAug 6, 2013 · Note: Make sure that you use the Click‑iT® reaction wash buffer 2 (Component J) for the. wash, and not the Click‑iT® reaction wash buffer 1 (Component I). 5.3 After the final wash, add 300 μL of Click‑iT® reaction wash buffer 2 (Component J) along the. inside of the tube where the beads are collected to resuspend the beads in the … WebThe Click-iT Cell Buffer Kit provides researchers who have cells labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two moieties together. exponential vs logical growth