Dna replicons for plant genome engineering
Web10 rows · DNA Replicons for Plant Genome Engineering. Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas ... WebJan 17, 2014 · Abstract. Sequence-specific nucleases enable facile editing of higher eukaryotic genomic DNA; however, targeted modification of plant genomes remains …
Dna replicons for plant genome engineering
Did you know?
WebThese observations, together with the absence of significant genetic changes in their core genome, suggest an ongoing flow of both mobile elements and E. coli lineages between human and natural ecosystems. ... This was not surprising, since these conjugative replicons are well-known to have higher prevalence in E. coli, in part due to the lower ... WebCRISPR/Cas is in the process of inducing the biggest transformation of plant breeding since the green revolution. ... of inducing directed changes by homologous recombination have been improved significantly and strategies to break genetic linkages by inducing chromosomal rearrangements have been developed. Cas13 systems have been applied …
Webor physical means to deliver genome engineering reagents (Cai et al., 2009; Shukla et al., 2009); however, the highest frequencies of gene targeting have been achieved using … WebDec 31, 2013 · DNA Replicons for Plant Genome Engineering. TL;DR: The feasibility of using geminivirus replicons to generate plants with a desired DNA sequence …
WebApr 17, 2024 · The DNA viruses belonging to the geminivirus class have been used to deliver gene-editing reagents in plants. The advantage of these viruses is that their single-stranded genome is converted to a double-stranded intermediate by host DNA polymerases once they are inside a host cell's nucleus. Web8 rows · We demonstrate the feasibility of using geminivirus replicons to generate plants with a desired ...
Web(2014) Baltes et al. Plant Cell. Sequence-specific nucleases enable facile editing of higher eukaryotic genomic DNA; however, targeted modification of plant genomes remains …
National Center for Biotechnology Information google books for publishersWebFeb 28, 2014 · Abstract. Sequence-specific nucleases enable facile editing of higher eukaryotic genomic DNA; however, targeted modification of plant genomes remains … google books for windows 10WebGenomic DNA was extracted from leaves of seedlings using the Nucleon Phytopure extraction kit (Cytiva) according to the manufacturer's protocol. Two micrograms of rice genomic DNA or 15 μg of tobacco genomic DNA was digested with the same restriction enzyme as that used in CAPS analysis and fractionated in a 0.8% agarose gel. google books family libraryWebJan 17, 2014 · We demonstrate the feasibility of using geminivirus replicons to generate plants with a desired DNA sequence modification. … google books free downloaderWebDNA replicons for plant genome engineering. Baltes NJ et al. The Plant Cell. 2014 Jan; 26(1):151-163. ... These results, in addition to the large host range of geminiviruses, … googlebooks into the wildWebMar 18, 2024 · DNA replicons for plant genome engineering. Plant Cell. 2014; 26: 151-163. Crossref; PubMed; Scopus (332) Google Scholar). For this purpose, we modified the WDV2 vectors to contain a Ubiquitin promoter::Cas9::NOS terminator cassette outside the WDV replicon boundaries (named WDV3) (Supplemental Figure 12) and used them to … google books free to readWebApr 11, 2024 · The DNA is inserted into the plant cell nucleus where the single stranded viral DNA is converted into the double stranded DNA replicative form and RNA is transcribed. The engineered viral transcript … google books fair use