Web23 May 2024 · Activity-dependent and Inducible Tagging of Dorsal and Ventral Hippocampus. We first infused an inducible, activity-dependent virus cocktail of AAV9-c-Fos-tTA and AAV9-TRE-eYFP into the dorsal or ventral dentate gyrus (DG) of adult male mice, which labeled DG neurons expressing the immediate-early gene c-Fos in a … Web5 Jun 2013 · Fos, Arc, and other IEGs have been frequently used as markers for neurons that were active during a short period prior to sacrifice. Although no single IEG is a …
018306 - B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
Web8 Jan 2024 · Fos-tTa and WT male mice were used for chemogenetics experiments (Experiment 3). All mice were housed under a 12 h light/dark cycle (lights on at 7:00 A.M.) at the maintained temperature of 21 ± 1°C and 50 ± 5% relative humidity. Mice used in the Fos-GFP expression time course experiment were ∼20–25 weeks old at the time of testing. WebPlasmid pAAV-cFos-tTA-pA from Dr. William Wisden's lab contains the insert cfos promoter linked to the tet activator gene and is published in Nat Neurosci. 2015 Apr;18(4):553-61. … jedi judd star wars
A transgenic mouse line for collecting ribosome-bound mRNA …
Web4 Jun 2024 · Although the c-Fos-tTA system enabled us to identify and manipulate engram cells, there was no approach to investigate their patterns of activity. When a neuron is activated, Ca 2+ flows into the soma. This neuronal activity can be translated into green fluorescence of a Ca 2+ indicator, such as GCaMP [ 17, 18 ]. Web4 Apr 2024 · Tg (Fos-tTA)1Mmay. Mutation details : The c-fos-tTA transgene was designed with the promoter region from the mouse FBJ osteosarcoma oncogene (Fos or c-fos; at least 611 bp of 5' untranscribed sequence that encompasses the known mouse c-fos regulatory elements) placed upstream of the tetracycline-regulated transactivator gene … jedi journal galaxy's edge