Halo assay protocol
WebApr 14, 2024 · The functional expression of the purinergic halo in human pDCs described in this work opens new areas to investigate its participation in the regulatory pDC mechanisms in health and disease. ... Supernatants were thawed at 4 °C, and the IFN-α assay (Invitrogen ThermoFisher, detection limit 3.2 pg/mL) was done according to the … WebNov 1, 2024 · We identified 7-bromoheptanol as a high-affinity, low-toxicity HaloTag-blocking agent that permits protein turnover measurements at both the cell population (by blotting) and single-cell (by imaging) levels. We show that the HaloTag pulse-chase approach is a nontoxic alternative to inhibition of protein synthesis with cycloheximide and extend ...
Halo assay protocol
Did you know?
WebMay 20, 2024 · The FP assay is one of the most accessible assays that can be easily set up with various protocols available [36,37]. The fluorescent probe is the key for the FP assay, which can be synthesized by a wide range of commercial services and academic core facilities or can be prepared in-house by attaching a fluorophore to a ligand. WebDifferent versions of Comet Assay. 1. Alkaline Comet Assay. 2. Neutral Comet Assay. 3. Comet assay at pH 12.1. 4. Comet assay with DNA repair enzymes. 5. Acellular Comet Assay. 6. Comet-FISH Assay. 7. Bromodeoxyuridine Labeling to Detect Replicating DNA. 8. Detection of incomplete DNA excision repair sites. 9. Halo assay or non …
WebHaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis. Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you open a gateway to investigate protein function. Once your protein of interest ... WebHaloPlex HS Custom Kits offer amplicon capture-based target enrichment with ultra-high sensitivity. By tagging every molecule with an unique molecular barcode, and capturing DNA from both strands, HaloPlex HS enables confident detection of rare variants with significantly improved base calling accuracy. These kits utilize a fast workflow of as little as under 6 …
WebSep 5, 2006 · The fast halo assay: an improved method to quantify genomic DNA strand breakage at the single-cell level The present study describes the improvement of a … WebV (annexin V–FITC); this assay is combined with analysis of the exclusion of the plasma membrane integrity probe propidium iodide (PI; see Basic Protocol 5). Also described are methods of analysis of DNA fragmentation based either on DNA content of cells with fractional (sub-G 1) DNA content (see Basic Protocol 6 and Alternate Protocol 1) or by
WebHT-HS10. Halosperm ® is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm ® allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional …
Web1 µL. 1 µM. E3 Ligase. X µL iv. 1 µM. iiiThe volume needed will depend on the stock concentration of your substrate. ivThe volume needed will depend on the stock concentration of your E3 ligase. Incubate in a 37 °C water … is spokeo safe and legitWebJan 1, 2009 · The fast-halo assay (FHA) is a very recent method to detect DNA-strand breakage induced either by various genotoxic agents or secondary to apoptotic DNA cleavage, and to study the repair of primary DNA breaks at the single-cell level. ... Cite this protocol. Sestili, P. (2009). The Fast-Halo Assay for the Assessment of DNA Damage … is spokeo legit and safeWebThe Autophagy LC3 HiBiT Reporter Assay System uses a luminescent LC3 reporter to quantitatively measure autophagic flux. Choose from one of two prepared reporter cell lines (HEK293 or U2OS) or stably transfect the reporter vector into the cell line of your choice. With one assay, you can verify results using three different methods: plate ... ifit yearly subscriptionWebMar 30, 2015 · In microorganisms, and more particularly in yeasts, a standard phenotyping approach consists in the analysis of fitness by growth rate determination in different conditions. One growth assay that … if i type 45 wpm what is my kphWebInoculate overnight YPD cultures of the mating type testers. Dilute the overnights 1:10 with sterile media or water. Spread ~200 µl lawn on YPD plates, one for each … if i type 75 wpm what is my kphWebHaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many … is spokeo safe to purchaseWebThe HaloCHIP™ System is a novel system that covalently captures crosslinked protein:DNA complexes from cells without the need for antibodies, providing a robust and efficient … if i type 40 wpm what is my kph