Hct116 puromycin
WebThis Luciferase & GFP / human HCT116 cell line was transformed via Lentivirus transduction. It has the Luciferase and GFP was constitutively co-expressed under EF1a promoter, as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following … WebThis cell line stably expresses luciferase at high level. It also expresses a green fluorescent protein (GFP) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments. Cell Line: HCT116. Reporters: Luciferase, GFP. Selection Marker: Puromycin
Hct116 puromycin
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WebFeb 22, 2010 · For IL4Ra knockdown in HCT116 cells, ‘Sure-Silencing’ plasmids encoding shRNA sequences directed toward four different regions of human IL4Ra ... murine or human cells were placed under selection with puromycin (10 μg/ml) or hygromycin (200 μg/ml), respectively. Once stable populations were obtained, individual clones were … WebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram-positive bacteria and various animal and insect cells.
WebNational Center for Biotechnology Information WebApr 7, 2024 · In line with these, we observed protein levels of DDX21 in most of CRC cell lines, such as HCT116, LOVO and RKO, are significantly higher than those in normal colonic epithelial cell NCM460 except ...
WebApr 1, 2024 · After 72 h of transfection, 2 mg/ml puromycin was added to the culture medium for HCT116 or RKO selection, respectively. ... A HCT116 cells in the siHOXC6 group were more sensitive to 5-Fu, ... WebJan 10, 2024 · Briefly, growth media containing DMEM (high glucose, pyruvate, ThermoFisher Scientific, Waltham, MA, USA) for HeLa Kyoto and HCT116 cells and …
WebDec 30, 2024 · Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose product, puromycin acetyltransferase, inactivates puromycin via acetylation; recommended concentration in cell culture 1-10µg/ml. Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of …
WebProtocol: Puromycin, Blasticidin and Hygromycin Titration Brief Description: A lentiviral construct carrying an antibiotic resistance gene will allow selection of the cell population … nals committeeWebJan 1, 2016 · Homeowners aggrieved by their homeowners associations (HOAs) often quickly notice when the Board of Directors of the HOA fails to follow its own rules, or … nalsc.orgWebDec 18, 2024 · Antibiotics selection with puromycin and blasticidin S increased the efficiency to knock cassettes with selection markers into the target genomic regions (38.7% for 5′, 34.4% for 3′ blasticidin S... nalsa winter internshipsWebApr 24, 2024 · Notably, puromycin upregulated the expression of RPL5 and RPL11 to directly bind to MDM2 in HCT116 cells. Conversely, deletion of RPL5 and RPL11 … nals chicagoWebDec 22, 2005 · Transfected HCT116 cells were maintained in medium supplemented with 1 μg/mL puromycin (Invitrogen Life Technologies). Stable overexpression of c-FLIP was assessed by Western blot analysis. siRNA Transfections siRNAs were designed using the Invitrogen RNA interference designer tool 1 1 … nals campingWebApr 12, 2024 · Puromycin was added to the cells for 5 days and individual cell colonies were isolated. The knockout effect was examined by Western blot with DNMT1 antibody (clone EPR3521(2); Abcam) in the ... nals chaptersWebJun 4, 2024 · 26th Jun, 2024 Matt Brown Try using these kits, they're optimized for both cell lines and have been used in siRNA delivery with around 70% transfection efficiency. HCT116:... nals cpe