Lineage-specific genetic ablation
Nettet28. jun. 2009 · Tipping the balance of early cytokine production can lead to lineage bias and, potentially, immune-mediated pathology. Mapping of a leishmania-susceptibility region has identified a gene that may ... Nettet20. apr. 2024 · Specific genetic targeting of GPCMs is the basis for accurately evaluate the cell fate and function during heart repair and regeneration. ... A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods. 2005; 2:419–426. doi: 10.1038/nmeth762 Crossref Medline Google Scholar; 34.
Lineage-specific genetic ablation
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NettetTo enable more specific cell lineage tracing and ablation through dual recombinase activity, we generated two mouse lines that render Dre- or Dre+Cre-mediated … Nettet8. des. 2014 · Cell Ablations Can Confound Lineage Designations 662 Developmental Cell 31 , 654–667, December 8, 2014 ª 2014 Elsevier Inc. Using an extensive array of genetic approaches combined
Nettet5 timer siden · The proximity of the F-cell to the socket cell and the shaft cell suggests that F-cell specification may depend on cells in the SOP lineage. By ablating different combinations of tactile bristle ... NettetLineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene …
Nettet1. apr. 2024 · To enable more specific cell lineage tracing and ablation through dual recombinase activity, we generated two mouse lines that render Dre- or Dre+Cre-mediated recombination to excise a stop codon ... Nettet2. apr. 2024 · 1 Introduction. Single-cell RNA-sequencing (scRNA-seq) technologies offer a chance to understand the regulatory mechanisms at single-cell resolution (Wen and Tang 2024).Subsequent to the technological breakthroughs in scRNA-seq, several analytical tools have been developed and applied towards the investigation of scRNA-seq data …
Nettet1. jun. 2005 · Abstract. A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT). To streamline this approach, we generated Cre-inducible DTR transgenic mice (iDTR) in which Cre-mediated excision of a STOP cassette renders cells sensitive to DT.
Genetic ablation occurs when a gene is deemed “null” through the homologous genetic recombination of a gene. It is utilized in the selective suppression of a specific cell line or cell type. This genetic engineering technique does not limit growth suppression to just the activity of an individual gene. Specific cell … Se mer In 1990, the gene knockout technique was just developing. There was a lack of information on the initial events that occur throughout the development of the vertebrate embryo. In order to form a better understanding, the … Se mer Temporal control of gene expression and ablation can be attributed to evolving transgenic and gene-therapy technologies. These technologies are enhanced by an understanding of the … Se mer The ability to selectively remove cells by ablation is monumental in the study of the development of eukaryotic biology, contributing greatly to the study of the origin, fate, or function of the cells. Genetic ablation occurs through the delivery of a toxin or death … Se mer 厚揚げレシピ人気 1位NettetGene Deletion. Cell-specific gene deletion and genetic epistasis experiments has established that in vivo osteocalcin is the ligand of Gprc6a that explains the regulation … 厚 揚げ レシピ 人気 1位 チーズNettet29. jul. 2012 · Lineage tracing and genetic ablation of ADAM12(+) ... Notably, we defined the specific gene signatures for PMSCs and hepatic stellate cells (HSCs), respectively. 厚揚げ レシピ 人気 簡単 トースター