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Peasy-blunt e1

WebNov 14, 2024 · The amplified α-CA was ligated into the pEASY ®-Blunt E1 Expression Vector (TransGen Biotech) and then transformed into Trans1-T1 phage-resistant chemically competent cells (TransGen Biotech). The plasmid was extracted by E.Z.N.A.™ plasmid mini kit (D6943-01) (OMEGA), sequenced and transformed into Trans etta (DE3) chemically … WebpEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. Pricing & Availability Download Catalogue Add to wishlist. View More Details Add to Cart.

Biogenesis of flavor-related linalool is diverged and genetically ...

WebFeb 28, 2024 · E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in normal liquid LB medium overnight, then supplemented with 50 μM CdCl 2 (b), 100 μM CdCl 2 (c), 200 μM ZnCl 2 (e) and normal LB (a, d). The cells were incubated at 37 °C for 10 h and 20 h. WebpEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. how many miles do planes fly https://blahblahcreative.com

Molecular characterization and expression analysis of

WebPeasy Blunt E1 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebAug 26, 2024 · After sequence confirmation by Sangon Biotechnology (Shanghai, China), the positive recombinant plasmids were designated as pEASY-Blunt E1-PiGSTd1. PiGSTd1 … WebSDS-PAGE gel electrophoresis of BoaZDS expressed in E. coli M: Protein marker (6.5-200 kD); 1: Non-induced control; 2: Control induced for 8 h; 3,5,7: Non-induced expression products of pEASY... how are potato plants reproduce asexually

杜梨PbGID1家族基因克隆、CRISPR/Cas9载体构建及遗传转化

Category:Molecular Cloning and Expression Analysis of the

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Peasy-blunt e1

Overexpression of the glucosyltransferase gene - ScienceDirect

Web糙皮侧耳新831菌株为河南农业大学生命科学学院应用真菌研究室保藏；pEASY-blunt E1 expression kit、pEASY-blunt cloning kit、TransStart FastPfu DNA polymerase购自北京全式金生物技术有限公司；E.coliDH5α、E.coliBL21（DE3）购自北京博迈德基因技术有限公司；RNA trizol plus购自大连宝 ... WebAug 1, 2024 · The empty plasmid pEASY-Blunt-E1was transformed into DE3 cells and served as control. The transformant BL21-CLEC4EL and the control cell were cultured in LB medium at 37 °C to mid-log, and induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG) at 37 °C for 4.5 h, respectively.

Peasy-blunt e1

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WebpEASY-Blunt Simple (linearized) Sequence and Map Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a … WebTransGen Biotech pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high …

WebJan 15, 2024 · In Pseudomonas aeruginosa PAO1, exogenous AI-2 influenced the biofilm amount and virulence factor expression in a dose-dependent manner in vitro and increased the histological lung damage in mice. 9 In Streptococcus suis, AI-2 supplemented exogenously had an impact on the expression of virulence genes and host–cell … WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA …

WebpEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 … Web快速：利用DNA拓扑异构酶I，室温5分钟，快速克隆。 简单：酶已偶联到载体上，只需加入片段即可。 高效：载体（已偶联酶）和片段双分子碰撞，连接成功率高。 pEASY® …

WebAug 28, 2024 · A positive colony harboring the recombinant plasmid was identified by colony PCR , confirmed by commercial sequencing and designated as pEASY-Blunt E1-ALDH. …

WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 how are potholes fixed in europeWebThe pEASY-Blunt E1-trxyn10 vector was trans-formed into E. coli BL21 (DE3), and the positive clones were isolated for TrXyn10 expression. The transformants were cultured overnight in LB culture medium with 100 lg/ml ampicillin at 37 C and 220 rpm. Then, 1 ml of the cells was added to 100 ml of LB medium at 25 C and 220 rpm. During culti- how are potatoes good for the bodyWebpEASY® -Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient … how are potholes badWebJul 1, 2024 · Under the conditions, IPTG 0.5 mmol/L, 16 °C, and overnight, recombinant protein of BcbZIP134 was obtained in high content using pEASY®-Blunt E1 vector and Transetta (DE3) E. coli. Anti-serum against BcbZIP134 was obtained with a high titer of 1: 51 200 after immunization in rabbits. how are pot gummies madeWebA positive colony harboring the recombinant plasmid was identified by colony PCR [41], confirmed by commercial sequencing and designated as pEASY-Blunt E1-ALDH. pEASY-Blunt E1-ALDH was then... how are potatoes planted commerciallyWebMay 1, 2024 · The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. Upon reaching an OD 600 of 0.6–0.8, expression in the transformed cells was induced using 1 mmol·L −1 isopropyl β - d-1-thiogalactopyranoside (IPTG), and the proteins were collected at 8 h after induction. how are potato plants propagated quizletWebMay 1, 2024 · The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. Upon reaching an OD 600 of 0.6–0.8, expression in the … how are potatoes consumed in the us