WebA signal peptide (sometimes referred to as signal sequence, targeting signal, localization signal, localization sequence, transit peptide, leader sequence or leader peptide) is a short … WebNov 25, 1997 · The expression plasmids for IL-1RAcP and AcP(1–403) were constructed by inserting PCR-generated cDNA fragments lacking coding sequence for the signal peptide into the mammalian expression vector pFlag-CMV-1 (Kodak), which contains the preprotrypsin leader sequence for transient expression of amino-terminal Flag fusion …
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WebWe replaced the original bacterial signal peptide with the preprotrypsin leader sequence to ensure the efficient secretion from mammalian cells and attached a Myc tag at the C-terminus to facilitate the detection of the ... The enzyme was engineered for secretion from HEK293T cells by the addition of preprotrypsin sequence to the N-terminus. WebVectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG® antibodies, resins, and plates. 9. pCMV6 Mammalian Expression Vectors– Physical Maps of pCMV6-XL4 , pCMV6-XL5 and pCMV6-XL6 10. dr brazeal
p3xFLAG-Myc-CMV-23 Sequence and Map - snapgene.com
WebJun 3, 2010 · The upstream preprotrypsin leader sequence was deleted from pFlag-TL1A-FL by site directed mutagenesis using the primers 5′-cgtcagaattaattcaccatg-3′ (forward) and 5′-gtcgtcatcgtctttgtagtc-3′ (reverse). To prepare TL1A-FL expression vector, Flag … WebApr 18, 2010 · The upstream preprotrypsin leader sequence was deleted from pFlag-TL1A-FL by site directed mutagenesis using the primers 5′-cgtcagaattaattcaccatg-3′ (forward) and 5′-gtcgtcatcgtctttgtagtc-3′ (reverse). To prepare TL1A-FL … WebProduct: leader sequence from mouse preprotrypsin: preprotrypsin leader 932 .. 976 = 45 bp 15 amino acids = 1.4 kDa. Product: leader sequence from mouse preprotrypsin: … dr brazee